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kanr addgene pet28a llds expression plasmid  (Addgene inc)


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    Addgene inc kanr addgene pet28a llds expression plasmid
    Kanr Addgene Pet28a Llds Expression Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 103 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/kanr addgene pet28a llds expression plasmid/product/Addgene inc
    Average 94 stars, based on 103 article reviews
    kanr addgene pet28a llds expression plasmid - by Bioz Stars, 2026-04
    94/100 stars

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    Expression of CCPs in diverse gram-negative bacteria. Expression of fluorescent proteins from the pKEK-Chrom-p15a-FnOri plasmid series in E. coli , V. alginolyticus , and S. oneidensis harboring expression vectors for eforCP (pKEK3193), DasherGFP (pKEK3194), tsPurple (pKEK3199), aeBlue (pKEK3198), YukonOFP (pKEK3197), sfGFP (pKEK3220), and sfCherry2 (pKEK3221). (A) Strains grown on agar plates and visualized under white (“W”), red (“UV-Red”), or green (“Blue-green”) filter. (B) Pellets of Escherichia coli strains from panel A grown in liquid media. (C) E. coli strains from panel A expressing sfGFP, DasherGFP, sfCherry2, and eforCP visualized via fluorescence microscopy. Created in BioRender.

    Journal: Applied and Environmental Microbiology

    Article Title: Mobilizable shuttle vectors with fluorescent markers functional across different species of bacteria

    doi: 10.1128/aem.00045-25

    Figure Lengend Snippet: Expression of CCPs in diverse gram-negative bacteria. Expression of fluorescent proteins from the pKEK-Chrom-p15a-FnOri plasmid series in E. coli , V. alginolyticus , and S. oneidensis harboring expression vectors for eforCP (pKEK3193), DasherGFP (pKEK3194), tsPurple (pKEK3199), aeBlue (pKEK3198), YukonOFP (pKEK3197), sfGFP (pKEK3220), and sfCherry2 (pKEK3221). (A) Strains grown on agar plates and visualized under white (“W”), red (“UV-Red”), or green (“Blue-green”) filter. (B) Pellets of Escherichia coli strains from panel A grown in liquid media. (C) E. coli strains from panel A expressing sfGFP, DasherGFP, sfCherry2, and eforCP visualized via fluorescence microscopy. Created in BioRender.

    Article Snippet: The pKEK-Chrom-RSF1010-p15a-KanR plasmids ( ) were mobilized into A. baumannii via conjugation.

    Techniques: Expressing, Bacteria, Plasmid Preparation, Fluorescence, Microscopy

    Alternate ori RSF1010 broadens host range and facilitates CCP expression in A. baumannii . (A) Schematic showing the plasmid structure of the pKEK-Chrom-RSF1010-p15a plasmid set evaluated in A. baumannii . (B) A. baumannii strains harboring plasmids expressing eforCP (pKEK3273), DasherGFP (pKEK3225), tsPurple (pKEK3226), aeblue (pKEK3228), Yukon (pKEK3227), sfGFP (pKEK3237), and sfCherry2 (pKEK3238), as well as A. baumannii with no fluorescent protein (“−”) were grown on agar plates and visualized under white (“W”), red (“UV-Red”), or green (“Blue-green”) filter. FP = fluorescent protein. Created in BioRender.

    Journal: Applied and Environmental Microbiology

    Article Title: Mobilizable shuttle vectors with fluorescent markers functional across different species of bacteria

    doi: 10.1128/aem.00045-25

    Figure Lengend Snippet: Alternate ori RSF1010 broadens host range and facilitates CCP expression in A. baumannii . (A) Schematic showing the plasmid structure of the pKEK-Chrom-RSF1010-p15a plasmid set evaluated in A. baumannii . (B) A. baumannii strains harboring plasmids expressing eforCP (pKEK3273), DasherGFP (pKEK3225), tsPurple (pKEK3226), aeblue (pKEK3228), Yukon (pKEK3227), sfGFP (pKEK3237), and sfCherry2 (pKEK3238), as well as A. baumannii with no fluorescent protein (“−”) were grown on agar plates and visualized under white (“W”), red (“UV-Red”), or green (“Blue-green”) filter. FP = fluorescent protein. Created in BioRender.

    Article Snippet: The pKEK-Chrom-RSF1010-p15a-KanR plasmids ( ) were mobilized into A. baumannii via conjugation.

    Techniques: Expressing, Plasmid Preparation